Vol. 6, Issue 1 (2017)

Author(s):
VP Prysyazhnyuk, OI Voloshyn and LP Sydorchuk

Abstract:
Investigation of A313G polymorphism of GSTP1 gene in chronic nonviral hepatitis patients was performed. Prevalence of G-allele of A313G polymorphism in GSTP1 gene did not differ significantly in chronic hepatitis patients compared with healthy individuals (OR = 1,36, CI = 0,70 – 2,66, p<0,05). Сhronic hepatitis patients were characterized by elevated plasma content of total bilirubin and its fractions, triacylglycerols, uric acid, higher activity of hepatic transaminases, alkaline phosphatase and gamma-glutamyl transferase compared with proper parameters in healthy individuals regardless of allelic distribution of GSTP1 gene. Alanineaminotransferase activity was at 44,0% (p = 0,046) higher in chronic hepatitis patients G-allele carriers compared with proper indicator in patients with AA-genotype, pointing out a higher activity of cytolysis. Analysis of pro- and antioxidant indicators reviled that chronic hepatitis patients both AA-genotype and G-allele carriers of GSTP1 gene had lower restored glutathione level, higher glutathione peroxidase activity and higher reactive products of thiobarbituric acid content in the blood, compared to such indicators in the control group. Chronic hepatitis patients G-allele carriers also noted significantly lower catalase activity compared with healthy persons, which was not typical for patients with A-allele. Significantly lower restored glutathione blood content at 16,2% (p = 0,04) was found in patients with G-allele compared with patients AA-genotype carriers.