Vol. 5, Issue 3 (2016)

Author(s):
Larysa Sydorchuk, Oksana Bochkariova, Oksana Andriyets, Andriy Sydorchuk, Oksana Bodnariuk

Abstract:
The aim of the study was to analyze the cytokines levels depending on 1267А⟶G polymorphism of HSP70-2 gene in 70 juvenile age girls of Northern Bukovina (Western Ukraine) with vulvovaginitis. The homozygous mutation of HSP70-2 gene (rs1061581) in study group was found in 2.86% cases, and was absent in control. Wild G allele of HSP70-2 gene present in every second teenager. The vulvovaginitis course in juvenile girls is accompanied by an increase of cellular immunity activity with prevailing of high and moderate production of pro-inflammatory cytokines (IL-1β and TNFα) 2.89 times (𝒳²=33.03, Ñ€<0.001) (especially in the GG-genotype carriers by 12.13% (Ñ€=0.04) and 7.19 (Ñ€=0.055), respectively) with compensatory increase of humoral immunity activity by synthesis of anti-inflammatory IL-10 by 22.01% (Ñ€<0.001) and activation of local immunity of the vaginal mucosa by the secretory sIgA increase 2.6 times (Ñ€<0.001), which did not depend on allele condition of HSP70-2 gene. Genotypes and alleles of HSP70-2 gene (rs1061581) are not the additional risk factors of the vulvovaginitis in juvenile girls of Northern Bukovina.