Vol. 4, Issue 4 (2015)
In- vitro comparative study of Anti-inflammatory and anti-arthritic effects of the methanol extract of Cissus pentagona Roxb and Thunbergia grandiflora Roxb. Leaf
Mohammad Nazmul Alam, Md. Shahrear Biozid, Md. Rafikul Islam, Md. Masudur Rahman, Ahmad Ibtehaz Chowdhury, Muhammad Moin Uddin Mazumdar
Objective: To evaluate comparative study of anti-inflammatory, anti-arthritic activity of methanol extract of Cissus pentagona Roxb. (Vitaceae.) and Thunbergia grandifloraRoxb. (Acanthaceae) leaf.
Methods: Human Red Blood Cell (HRBC) membrane stabilization method was evaluated for anti-inflammatory activity. Anti-denaturation method was performed by using bovine serum albumin (BSA) to evaluate the anti-arthritic potential.
Results: The in vitro anti-inflammatory activity of the methanol extracts of C. pentagona andT. grandiflora showed 78.89% and 89.11% of membrane stabilization at 1000 µg/ml conc and 42.22% and 63.37% at 31.25 µg/ml respectively. All the results were compared with standard Diclofenac which showed 94.44% protection at 1000 µg/ml conc. The in vitro study on both leaves also showed the presence of significant anti-arthritic activity. Here the extracts of C. pentagona and T. grandiflora showed 69.35% and 74.19% of protein denaturation at highest conc (1000 ug/ml) and 38.71% and 45.16% at lowest conc (31.25 ug/ml) respectively, in where the standard drug showed the 85.49% at 1000 ug/ml and 51.61% at 31.25 ug/ml conc.Conclusion: These result suggested that both the methanol extract of C. pentagona Roxb. and T. grandiflora Roxb. contains anti- inflammatory and anti-arthritic activity.
How to cite this article:
Mohammad Nazmul Alam, Md. Shahrear Biozid, Md. Rafikul Islam, Md. Masudur Rahman, Ahmad Ibtehaz Chowdhury, Muhammad Moin Uddin Mazumdar. . The Pharma Innovation Journal. 2015; 4(4): 39-42.