Vol. 1, Issue 9 (2012)
Direct Shoots Regeneration from Nodal Meristems of Brugmansia suaveolens (Humb. & Bonpl. ex Willd.) Bercht. & J.Presl.
Dr. M.S. Shekhawat
An efficient tissue culture protocol has been developed for micropropagation of Brugmansia suaveolens. The fresh sprouts were used as explants. These were surface sterilized. The explants were responded in 15 days after inoculation on Murashige and Skoog (MS) medium supplemented with 2.0 mgl-1 Benzylaminopurine (BAP). 5-6 shoots were regenerated on this media combination. Callus was formed when auxins were added to the medium. Maximum numbers of shoots (8-9 shoots/culture) were reported on MS medium augmented with 0.5 mgl-1 each of BAP and Kinetin. The long and healthy shoots were excised and isolated from the shoot clumps and used for in vitro rooting experiments. Indole-3 acetic acid (IAA) and Indole-3 butyric acid (IBA) both are equally effective in root induction. The in vitro rooted plantlets were hardened in green house in pots which contains mixture of soil and manure.