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Vol. 1, Issue 7 (2012)

Method Development and Validation of Neverapine by Rp-Hplc

Author(s):
Sindhu Reddy M*, Ramya Tulasi G, Neeharika G, Swetha M, Agilandeswari D, Dabhi Harshkumar D, Sajani Raju, Vineeth Chandy
Abstract:
A simple and accurate RP-HPLC method has been developed for the estimation of Neverapine in bulk and pharmaceutical dosage forms, using C-18 column 150 x 4.6 mm i.d, 5μm particle size in isocratic mode, with mobile phase comprising of phosphate buffer (pH 3) and Acetonitrile in the ratio of 40:60 v/v. The flow rate was 0.8ml/min and the detection was monitored out by UV detector at 270 nm. The retention time for Neverapine was found to be 3.308 min. The proposed method has permitted the quantification of Neverapine over linearity in the range of 20-60 μg/ml and its percentage recovery was found to be 101.7%.The intraday and inter day precision were found 0.06% and 0.13%, respectively.
Pages: 08-13  |  533 Views  4 Downloads
How to cite this article:
Sindhu Reddy M*, Ramya Tulasi G, Neeharika G, Swetha M, Agilandeswari D, Dabhi Harshkumar D, Sajani Raju, Vineeth Chandy. Method Development and Validation of Neverapine by Rp-Hplc. 2012; 1(7): 08-13.
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